In our CBCS Uppsala node we offer morphological profiling using Cell Painting as a service, including Cell Painting protocol optimization for a given cell line, Cell Painting experiments carried out using our automated lab, the necessary cell culture work, as well as automated imaging, image and data analysis, and visualization.

Services at CBCS Uppsala

Phenotypic Profiling Using Cell Painting

Phenotypic profiling of cells by high-throughput high-content microscopy allows for the identification of morphological changes in the cell that are indicative, for instance, of the mode of action (MoA) of a drug, novel compound, or biological.

Morphological changes in the cell can be measured using two major approaches: 1) making use of image analysis software (e.g. Cell Profiler) to identify and segment the cells, and subsequently extract selected cellular features, and 2) using Deep Learning (DL) to extract relevant cellular features in an unbiased manner. Upon the extraction and selection of the morphological features, morphological profiles are generated that are unique per each cell type or perturbation. This is achieved through extensive analysis of the vast number of features extracted at a single cell level.

Our node has extensive experience in large-scale data handling and analysis, and we have built a complete IT infrastructure capable of storing and analyzing large quantities of images and subsequent data generated from Cell Painting experiments.  We offer morphological profiling using Cell Painting as a service, including:

• Cell Painting protocol optimization for a given cell line.

• Cell Painting experiments were carried out using our automated lab.

• The necessary cell culture work.

• Automated imaging, image and data analysis, and visualization.